epifluorescence setup Search Results


epi-fluorescence microscopy set-up  (IonOptix)
90
IonOptix epi-fluorescence microscopy set-up
Epi Fluorescence Microscopy Set Up, supplied by IonOptix, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/epifluorescence+setup/pmc03670593-114-25-28?v=IonOptix
Average 90 stars, based on 1 article reviews
epi-fluorescence microscopy set-up - by Bioz Stars, 2026-08
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epifluorescence/spinning disk confocal setup  (VISITRON Inc)
90
VISITRON Inc epifluorescence/spinning disk confocal setup
A. AFM performed on MDCK cells cultured in 2D. (Left) Example of <t>epifluorescence</t> microscopy image; cells harboring structural centrosome aberrations (GFP-NLP) are marked (asterisks). The scan area (red square) and position of the AFM probe (red dot) are monitored in the brightfield signal for each experiment. (Middle) Corresponding stiffness map visualizes local nanomechanical heterogeneities; cells overexpressing GFP-NLP, as visualized by epifluorescence microscopy, are marked by asterisks. (Right) Stiffness ratios compare cells overexpressing NLP, PLK4 or CEP68 with neighboring non-expressing cells (WT); NLP-over-expressing cells are stiffer than WT cells while cells over-expressing either PLK4 or CEP68 exhibit mechanical phenotypes similar to WT cells. B. AFM performed on 3D MDCK cysts. (Left) brightfield image showing the AFM probe positioned above the isolated cyst. (Middle) stiffness map visualizes basal surface of individual cells within top region of 3D cysts. (Right) quantitative analysis confirms that NLP over-expression induces cell stiffening in 3D. C. Drug-induced changes of cytoskeletal structures alter cellular stiffness of WT (-Dox) or GFPNLP + (+Dox) MDCK cells in 2D. D. Cell cycle dependence of cellular stiffness. Position of the AFM probe is monitored in the brightfield signal while mCardinal-H1 signal detection by epifluorescence allows identification of mitoses and interphase cells. Images are 90 × 90 µm. E. Quantitative AFM analysis of confluent MDCK cells in interphase or in mitosis, in comparison to surrounding cells in interphase that do not express GFP-NLP (WT). n indicates the number of analyzed cells. Box plots show the mean (square) and median (line); whiskers are s.d. and the box is s.e.m. Statistical significance was tested using a Mann-Whitney test. (*), (**) and (***) indicate P-value <0.05, <0.01 and <0.005 respectively.
Epifluorescence/Spinning Disk Confocal Setup, supplied by VISITRON Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/epifluorescence+setup/bio_rxiv__216804-195-31-35?v=VISITRON+Inc
Average 90 stars, based on 1 article reviews
epifluorescence/spinning disk confocal setup - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier

Image Search Results


A. AFM performed on MDCK cells cultured in 2D. (Left) Example of epifluorescence microscopy image; cells harboring structural centrosome aberrations (GFP-NLP) are marked (asterisks). The scan area (red square) and position of the AFM probe (red dot) are monitored in the brightfield signal for each experiment. (Middle) Corresponding stiffness map visualizes local nanomechanical heterogeneities; cells overexpressing GFP-NLP, as visualized by epifluorescence microscopy, are marked by asterisks. (Right) Stiffness ratios compare cells overexpressing NLP, PLK4 or CEP68 with neighboring non-expressing cells (WT); NLP-over-expressing cells are stiffer than WT cells while cells over-expressing either PLK4 or CEP68 exhibit mechanical phenotypes similar to WT cells. B. AFM performed on 3D MDCK cysts. (Left) brightfield image showing the AFM probe positioned above the isolated cyst. (Middle) stiffness map visualizes basal surface of individual cells within top region of 3D cysts. (Right) quantitative analysis confirms that NLP over-expression induces cell stiffening in 3D. C. Drug-induced changes of cytoskeletal structures alter cellular stiffness of WT (-Dox) or GFPNLP + (+Dox) MDCK cells in 2D. D. Cell cycle dependence of cellular stiffness. Position of the AFM probe is monitored in the brightfield signal while mCardinal-H1 signal detection by epifluorescence allows identification of mitoses and interphase cells. Images are 90 × 90 µm. E. Quantitative AFM analysis of confluent MDCK cells in interphase or in mitosis, in comparison to surrounding cells in interphase that do not express GFP-NLP (WT). n indicates the number of analyzed cells. Box plots show the mean (square) and median (line); whiskers are s.d. and the box is s.e.m. Statistical significance was tested using a Mann-Whitney test. (*), (**) and (***) indicate P-value <0.05, <0.01 and <0.005 respectively.

Journal: bioRxiv

Article Title: Structural centrosome aberrations promote non-cell-autonomous invasiveness

doi: 10.1101/216804

Figure Lengend Snippet: A. AFM performed on MDCK cells cultured in 2D. (Left) Example of epifluorescence microscopy image; cells harboring structural centrosome aberrations (GFP-NLP) are marked (asterisks). The scan area (red square) and position of the AFM probe (red dot) are monitored in the brightfield signal for each experiment. (Middle) Corresponding stiffness map visualizes local nanomechanical heterogeneities; cells overexpressing GFP-NLP, as visualized by epifluorescence microscopy, are marked by asterisks. (Right) Stiffness ratios compare cells overexpressing NLP, PLK4 or CEP68 with neighboring non-expressing cells (WT); NLP-over-expressing cells are stiffer than WT cells while cells over-expressing either PLK4 or CEP68 exhibit mechanical phenotypes similar to WT cells. B. AFM performed on 3D MDCK cysts. (Left) brightfield image showing the AFM probe positioned above the isolated cyst. (Middle) stiffness map visualizes basal surface of individual cells within top region of 3D cysts. (Right) quantitative analysis confirms that NLP over-expression induces cell stiffening in 3D. C. Drug-induced changes of cytoskeletal structures alter cellular stiffness of WT (-Dox) or GFPNLP + (+Dox) MDCK cells in 2D. D. Cell cycle dependence of cellular stiffness. Position of the AFM probe is monitored in the brightfield signal while mCardinal-H1 signal detection by epifluorescence allows identification of mitoses and interphase cells. Images are 90 × 90 µm. E. Quantitative AFM analysis of confluent MDCK cells in interphase or in mitosis, in comparison to surrounding cells in interphase that do not express GFP-NLP (WT). n indicates the number of analyzed cells. Box plots show the mean (square) and median (line); whiskers are s.d. and the box is s.e.m. Statistical significance was tested using a Mann-Whitney test. (*), (**) and (***) indicate P-value <0.05, <0.01 and <0.005 respectively.

Article Snippet: All AFM experiments were carried out under close to physiological conditions using a customized mechano-optical microscope (MOM) comprised of an AFM (JPK Instruments AG, Germany and SPECS Zurich GmbH, Switzerland) and epifluorescence/spinning disk confocal setup (Visitron Systems GmbH, Germany).

Techniques: Cell Culture, Epifluorescence Microscopy, Expressing, Isolation, Over Expression, MANN-WHITNEY